Chromosoma

, Volume 103, Issue 9, pp 577–590

Well-defined genome architecture in the human sperm nucleus

Authors

  • A. O. Zalensky
    • Department of Biological ChemistryUniversity of California at Davis
  • M. J. Allen
    • Department of Biological ChemistryUniversity of California at Davis
    • Biology and Biotechnology Research ProgramLawrence Livermore National Laboratory
  • A. Kobayashi
    • Biology and Biotechnology Research ProgramLawrence Livermore National Laboratory
  • I. A. Zalenskaya
    • Department of Biological ChemistryUniversity of California at Davis
  • R. Balhorn
    • Biology and Biotechnology Research ProgramLawrence Livermore National Laboratory
  • E. M. Bradbury
    • Department of Biological ChemistryUniversity of California at Davis
    • Los Alamos National Laboratory
Article

DOI: 10.1007/BF00357684

Cite this article as:
Zalensky, A.O., Allen, M.J., Kobayashi, A. et al. Chromosoma (1995) 103: 577. doi:10.1007/BF00357684

Abstract

Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of centromere and telomere regions of all chromosomes and supported by localization of subtelomere sequences of chromosome 3 and the entire chromosome 2. The nuclear architecture in the human sperm is characterized by the clustering of the 23 centromeres into a compact chromocenter positioned well inside the nucleus. The ends of the chromosomes are exposed to the nuclear periphery where both the subtelomere and the telomere sequences of the chromosome arms are joined into dimers. Thus chromosomes in the human sperm nucleus are looped into a hairpin-like configuration. The biological implications of this nuclear architecture in spermatogenesis and male pronuclear formation following fertilization are discussed.

Copyright information

© Springer-Verlag 1995