Original Articles

Current Genetics

, Volume 25, Issue 3, pp 196-201

First online:

Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae

  • F. BidardAffiliated withLaboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.), INRA
  • , B. BlondinAffiliated withChaire de Technologie Alimentaire et Oenologie, Institut des Produits de la Vigne (I.P.V.), ENSA.M
  • , S. DequinAffiliated withLaboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.), INRA
  • , F. VezinhetAffiliated withLaboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.), INRA
  • , P. BarreAffiliated withLaboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.), INRA

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A yeast flocculation gene was isolated from a genomic library of an FLO5 strain of S. cerevisiae on the basis of its ability to trigger flocculation in a non-flocculent strain. Characterization of the cloned gene by restriction mapping, Southern analysis, and chromosome mapping have shown that it corresponds to a FLO5 gene previously located on chromosome I and that this gene is related to the already described. FLO1 gene. A study of gene expression in different yeast strains has indicated that, while this gene is dominant, its expression can be suppressed in some genetic backgrounds. A Northern-blot analysis has demonstrated that the same 5000-nt transcript was present in an FLO5 and an FLO1 strain. A gene disruption experiment has led to the conclusion that another flocculation gene is present and can be active in the FLO5 strain we used.

Key words

Yeast Flocculation Cloning Expression