Current Genetics

, Volume 25, Issue 3, pp 196–201

Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae

  • F. Bidard
  • B. Blondin
  • S. Dequin
  • F. Vezinhet
  • P. Barre
Original Articles

DOI: 10.1007/BF00357162

Cite this article as:
Bidard, F., Blondin, B., Dequin, S. et al. Curr Genet (1994) 25: 196. doi:10.1007/BF00357162

Abstract

A yeast flocculation gene was isolated from a genomic library of an FLO5 strain of S. cerevisiae on the basis of its ability to trigger flocculation in a non-flocculent strain. Characterization of the cloned gene by restriction mapping, Southern analysis, and chromosome mapping have shown that it corresponds to a FLO5 gene previously located on chromosome I and that this gene is related to the already described. FLO1 gene. A study of gene expression in different yeast strains has indicated that, while this gene is dominant, its expression can be suppressed in some genetic backgrounds. A Northern-blot analysis has demonstrated that the same 5000-nt transcript was present in an FLO5 and an FLO1 strain. A gene disruption experiment has led to the conclusion that another flocculation gene is present and can be active in the FLO5 strain we used.

Key words

Yeast Flocculation Cloning Expression 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • F. Bidard
    • 1
  • B. Blondin
    • 2
  • S. Dequin
    • 1
  • F. Vezinhet
    • 1
  • P. Barre
    • 1
  1. 1.Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne (I.P.V.)INRAMontpellier Cedex 1France
  2. 2.Chaire de Technologie Alimentaire et Oenologie, Institut des Produits de la Vigne (I.P.V.)ENSA.MMontpellier Cedex 1France