Molecular and General Genetics MGG

, Volume 184, Issue 3, pp 536–538

Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the β subunit gene of the rifampicin resistant rpoB255 mutant

  • Yu. A. Ovchinnikov
  • G. S. Monastyrskaya
  • V. V. Gubanov
  • V. M. Lipkin
  • E. D. Sverdlov
  • I. F. Kiver
  • I. A. Bass
  • S. Z. Mindlin
  • O. N. Danilevskaya
  • R. B. Khesin
Article

DOI: 10.1007/BF00352535

Cite this article as:
Ovchinnikov, Y.A., Monastyrskaya, G.S., Gubanov, V.V. et al. Mol Gen Genet (1981) 184: 536. doi:10.1007/BF00352535

Summary

The transducing phage λ dsupM814 and the plasmid pIB1830 containing the wild-type rpoB gene have been constructed and the primary structure of the gene's central fragment has been established. In contrast with the wild-type, the gene of the rpoB255 mutant, whose primary structure has been published, was found to contain an A.T.→T.A. transversion entailing the substitution of a valine residue for the aspartic acid residue (516) of the wild-type β subunit.

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Yu. A. Ovchinnikov
    • 1
  • G. S. Monastyrskaya
    • 1
  • V. V. Gubanov
    • 1
  • V. M. Lipkin
    • 1
  • E. D. Sverdlov
    • 1
  • I. F. Kiver
    • 2
  • I. A. Bass
    • 2
  • S. Z. Mindlin
    • 2
  • O. N. Danilevskaya
    • 2
  • R. B. Khesin
    • 2
  1. 1.Shemyakin Institute of Bioorganic ChemistryUSSR Academy of SciencesMoscowUSSR
  2. 2.Institute of Molecular GeneticsUSSR Academy of SciencesMoscowUSSR