Current Genetics

, Volume 27, Issue 4, pp 387–389

Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box

  • Olivier Vincent
  • Juana M. Gancedo
Short Communication

DOI: 10.1007/BF00352109

Cite this article as:
Vincent, O. & Gancedo, J.M. Curr Genet (1995) 27: 387. doi:10.1007/BF00352109

Abstract

We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block β-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.

Key words

Saccharomyces cerevisiae URS FBP1 Transcription 

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Olivier Vincent
    • 1
  • Juana M. Gancedo
    • 1
  1. 1.Arturo Duperior, no. 4Instituto de Investigaciones Biomédicas del C.S.I.C.MadridSpain