Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box
- Cite this article as:
- Vincent, O. & Gancedo, J.M. Curr Genet (1995) 27: 387. doi:10.1007/BF00352109
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We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYC1-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYC1-lacZ fusion gene can block β-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.