Current Genetics

, Volume 27, Issue 4, pp 320–329

Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals

  • Rajendra Prasad
  • Philippe De Wergifosse
  • Andre Goffeau
  • Elisabetta Balzi
Original Paper

DOI: 10.1007/BF00352101

Cite this article as:
Prasad, R., De Wergifosse, P., Goffeau, A. et al. Curr Genet (1995) 27: 320. doi:10.1007/BF00352101

Abstract

By functional complementation of a PDR5 null mutant of Saccharomyces cervisiae, we have cloned and sequenced the multidrug-resistance gene CDR1 of Candida albicans. Transformation by CDR1 of a PDR5-disrupted host hypersensitive to cycloheximide and chloramphenicol resulted in resistance to cycloheximide, chloramphenicol and other drugs, such as the antifungal miconazole, with collateral hypersensitivity to oligomycin, nystatin and 2,4 dinitrophenol. Our results also demonstrate the presence of several PDR5 complementing genes in C. albicans, displaying multidrug-resistance patterns different from PDR5 and CDR1. The nucleotide sequence of CDR1 revealed that, like PDR5, it encodes a putative membrane pump belonging to the ABC (ATP-binding cassette) superfamily. CDR1 encodes a 1501-residue protein of 169.9 kDa whose predicted structural organization is characterized by two homologous halves, each comprising a hydrophobic region with a set of six transmembrane stretches, preceded by a hydrophilic nucleotide binding fold.

Key words

Multidrug resistanceCandida albicansSaccharomyces cerevisiaeABC transporters

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Rajendra Prasad
    • 1
  • Philippe De Wergifosse
    • 1
  • Andre Goffeau
    • 1
  • Elisabetta Balzi
    • 1
  1. 1.Unite de Biochimie PhysiologiqueUniversite Catholique de LouvainLouvain-la-NeuveBelgium
  2. 2.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia