Current Genetics

, Volume 23, Issue 2, pp 181–183

Epitope-tagging vectors designed for yeast

  • Philippe Reisdorf
  • Ammy C. Maarse
  • Bertrand Daignan-Fornier
Short Communications

DOI: 10.1007/BF00352019

Cite this article as:
Reisdorf, P., Maarse, A.C. & Daignan-Fornier, B. Curr Genet (1993) 23: 181. doi:10.1007/BF00352019

Abstract

In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3′ end of any gene. An example of the use of this technique is presented.

Key words

Saccharomyces cerevisiaec-myc epitopeFusion

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Philippe Reisdorf
    • 1
  • Ammy C. Maarse
    • 2
  • Bertrand Daignan-Fornier
    • 1
  1. 1.Laboratoire de Génétique MoléculaireUniversité Paris-SudOrsay cedexFrance
  2. 2.Section for Molecular Biology, Department of Molecular Cell BiologyUniversity of AmsterdamAmsterdamThe Netherlands