Current Genetics

, Volume 24, Issue 5, pp 377–381

The ogd1 and kgd1 mutants lacking 2-oxoglutarate dehydrogenase activity in yeast are allelic and can be differentiated by the cloned amber suppressor

  • Daniela Mockovčiaková
  • Vanda Janitorová
  • Mária Zigová
  • Eva Kaclíková
  • Marek Zagulski
  • Július Šubík
Original Articles

DOI: 10.1007/BF00351844

Cite this article as:
Mockovčiaková, D., Janitorová, V., Zigová, M. et al. Curr Genet (1993) 24: 377. doi:10.1007/BF00351844

Abstract

The activity of mitochondrial 2-oxoglutarate dehydrogenase in S. cerevisiae can be impaired either by the ogd1 or the kgd1 mutation. The OGD1 gene and two suppressor genes were isolated by complementation of the ogd1 mutant. The complementation of the kdg1 mutant by the OGD1 gene, an allelism test, and meiotic mapping, revealed that the ogd1 and kgd1 mutations are allelic. The two mutations were differentiated by the cloned suppressor gene which was able to partially complement ogd1, but not kgd1. The molecular analysis of the suppressor gene revealed its identity with the natural tRNACAGGlngene found in the upstream region of URA10.

Key words

2-Oxoglutarate dehydrogenaseMolecular cloningSaccharomyces cerevisiaeSequencingSuppressorYeast

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Daniela Mockovčiaková
    • 1
  • Vanda Janitorová
    • 1
  • Mária Zigová
    • 2
  • Eva Kaclíková
    • 2
  • Marek Zagulski
    • 3
  • Július Šubík
    • 1
  1. 1.Department of Microbiology and VirologyComenius UniversityBratislavaSlovak Republic
  2. 2.Food Research InstituteBratislavaSlovak Republic
  3. 3.Institute of Biochemistry and BiophysicsPolish Academy of SciencesWarszawaPoland