Current Genetics

, Volume 22, Issue 3, pp 175–180

Mutational analysis of a variant of ARS1 from Saccharomyces cerevisiae

  • Finn Kirpekar
  • Kay Gulløv
Original Articles

DOI: 10.1007/BF00351723

Cite this article as:
Kirpekar, F. & Gulløv, K. Curr Genet (1992) 22: 175. doi:10.1007/BF00351723

Summary

A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the core consensus is deleted. However, deletion mutations revealed that this near-match together with a short palindromic sequence, also situated in the B-flanking region, comprise a single element crucial for optimal ARS function. The palindrome has the potential of forming a stemloop structure. Rather precise observations concerning the borders of the B-region were achieved. The four base pairs separating the near-match from the core consensus perform a spacing function where the identity of the bases are unimportant. However, this spacing is highly important since deletion of these four base pairs leads to an ARS- phenotype.

Key words

ARS1 mutantsDNA replicationYeastSingle-stranded DNA

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Finn Kirpekar
    • 1
  • Kay Gulløv
    • 1
  1. 1.Department of Molecular BiologyOdense UniversityOdense MDenmark