Original Articles

Current Genetics

, Volume 22, Issue 3, pp 175-180

Mutational analysis of a variant of ARS1 from Saccharomyces cerevisiae

  • Finn KirpekarAffiliated withDepartment of Molecular Biology, Odense University
  • , Kay GulløvAffiliated withDepartment of Molecular Biology, Odense University

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A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the core consensus is deleted. However, deletion mutations revealed that this near-match together with a short palindromic sequence, also situated in the B-flanking region, comprise a single element crucial for optimal ARS function. The palindrome has the potential of forming a stemloop structure. Rather precise observations concerning the borders of the B-region were achieved. The four base pairs separating the near-match from the core consensus perform a spacing function where the identity of the bases are unimportant. However, this spacing is highly important since deletion of these four base pairs leads to an ARS- phenotype.

Key words

ARS1 mutants DNA replication Yeast Single-stranded DNA