Original Articles

Current Genetics

, Volume 21, Issue 4, pp 345-349

First online:

Construction of a Tetracycline-inducible promoter in Schizosaccharomyces pombe

  • Khaschayar FaryarAffiliated withInstitut für Genbiologische Forschung Berlin GmbH
  • , Christiane GatzAffiliated withInstitut für Genbiologische Forschung Berlin GmbH

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Summary

We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.

Key words

CaMV 35S promoter Tet repressor Tetracycline induction Schizosaccharomyces pombe