Current Genetics

, Volume 21, Issue 4, pp 325–329

The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products

  • F. Bussereau
  • C. -H. Dupont
  • E. Boy-Marcotte
  • L. Mallet
  • M. Jacquet
Original Articles

DOI: 10.1007/BF00351690

Cite this article as:
Bussereau, F., Dupont, C.H., Boy-Marcotte, E. et al. Curr Genet (1992) 21: 325. doi:10.1007/BF00351690

Summary

The ccs1-1 mutation of Saccharomyces cerevisiae, which has been previously described, is associated with an increase in cytochrome content, in respiration, and in ATP synthesis. In addition, this mutation leads to the same phenotype as cells de-regulated in the cAMP pathway. From a yeast genomic library, we have isolated a DNA fragment in a recombinant plasmid pCD1 which complements the ccs1-1 mutation. Homologous integration of this DNA in the genome occurs at the CCS1 locus. An 11 kb of the DNA insert is necessary for complementation. Sequencing part of the fragment identifies CCS1 as the IRA2 gene. The IRA2 gene is known to encode an attenuator of RAS gene product activity which stimulates the GTPase activity of the RAS proteins. This result underlines the involvement of cAMP-dependent phosphorylation in mitochondrial function. We present the sequence of 1 kb DNA upstream of the putative ATG of the IRA2/CCS1 gene product which is devoid of an ORF and could contain several regulatory sites.

Key words

Yeast cAMP RAS GAP 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • F. Bussereau
    • 1
  • C. -H. Dupont
    • 1
  • E. Boy-Marcotte
    • 1
  • L. Mallet
    • 1
  • M. Jacquet
    • 1
  1. 1.Institut de Génétique et de Microbiologie, Laboratoire Information Génétique et Développement, U.R.A. 1354 du CNRSUniversité Paris-SudOrsay-cedexFrance