Current Genetics

, Volume 21, Issue 4, pp 295–300

Genetic analysis of serine biosynthesis and glucose repression in yeast

  • Karsten Melcher
  • Karl-Dieter Entian
Original Articles

DOI: 10.1007/BF00351686

Cite this article as:
Melcher, K. & Entian, KD. Curr Genet (1992) 21: 295. doi:10.1007/BF00351686


Serine and glycine biosynthesis in yeast proceed by two pathways; a “glycolytic” pathway, using 3-phosphoglycerate, and a “gluconeogenic” pathway, using glyoxylate. We used a mutation in the cat1 gene to abolish the glucose-repressible “gluconeogenic” pathway and re-isolated two mutants, ser1 and ser2, in the “glycolytic” pathway. The ser1 mutation corresponded to phosphoserine transaminase and ser2 to that of phosphoserine phosphatase. Mutagenesis of a ser1 ser2 cat1 triple mutant facilitated the isolation of a mutation in a new gene, SER10. SER10 appears to be part of a pathway which, under normal growth conditions, is less important in serine biosynthesis. The ser1 ser2 ser10 triple mutants were totally serine auxotrophic on glucose media but serine prototrophic during growth on non-fermentable carbon sources. This phenotype was used to select for possible regulatory mutants that synthesize serine by the gluconeogenic pathway even in the presence of glucose, e.g., with a non-glucose repressible glyoxylate cycle. In an alternative approach to isolate such mutants URA3 and TRP1 expression were placed under the control of the glucose-repressible FBP1 (fructose-1,6-bisphosphatase) promoter. Although both systems resulted in strong selection pressure we could not isolate constitutively derepressed mutants. These results indicate that transcription of glucose-repressible gluconeogenic enzymes is mainly dependent on positive regulatory elements.

Key words

Saccharomyces cerevisiae Serine biosynthesis Mutant isolation Glucose repression 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Karsten Melcher
    • 1
  • Karl-Dieter Entian
    • 1
  1. 1.Institut für MikrobiologieJ. W. Goethe-UniversitätFrankfurt/MainFederal Republic of Germany
  2. 2.University of Texas Southwestern Medical SchoolDallasUSA

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