Current Genetics

, Volume 23, Issue 3, pp 211–218

Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes

  • Howard S. Judelson
  • Michael D. Coffey
  • Felipe R. Arredondo
  • Brett M. Tyler
Original Articles

DOI: 10.1007/BF00351498

Cite this article as:
Judelson, H.S., Coffey, M.D., Arredondo, F.R. et al. Curr Genet (1993) 23: 211. doi:10.1007/BF00351498

Abstract

A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or complexed to cationic liposomes, was introduced into protoplasts using polyethylene glycol and CaCl2. DNA and RNA hybridization, and phosphotransferase assays, confirmed the presence and expression of vector DNA in the transformants. Hybridization to electrophoretically separated chromosomes of P. m. glycinea showed that vector DNA had integrated into only one chromosome in four transformants, and into multiple chromosomes in one transformant.

Key words

Transformation Oomycete Phytophthora megasperma Chromosome separationex 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Howard S. Judelson
    • 1
  • Michael D. Coffey
    • 3
  • Felipe R. Arredondo
    • 2
  • Brett M. Tyler
    • 2
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA
  2. 2.Department of Plant PathologyUniversity of CaliforniaDavisUSA
  3. 3.Department of Plant PathologyUniversity of CaliforniaRiversideUSA
  4. 4.Center for Engineering Plants for Resistance Against PathogensUniversity of CaliforniaDavisUSA

Personalised recommendations