Molecular Biology Reports

, Volume 23, Issue 3, pp 227–234

Primary biliary cirrhosis sera recognize not only gp210 but also proteins of the p62 complex bearing N-acetylglucosamine residues from rat liver nuclear envelope

Anti-p62 complex antibody in PBC
  • K. Miyachi
  • M. Shibata
  • Y. Onozuka
  • F. Kikuchi
  • N. Imai
  • T. Horigome
Features of Autoantigens

DOI: 10.1007/BF00351173

Cite this article as:
Miyachi, K., Shibata, M., Onozuka, Y. et al. Molecular Biology Reports (1996) 23: 227. doi:10.1007/BF00351173

Abstract

We have recently observed reactivity of primary biliary cirrhosis (PBC) sera with several proteins bearing N-acetylglucosamine residues from rat liver nuclear envelopes. The aim of this study was to characterize the reactive antigens. Sera from 31 patients with PBC, 30 with rheumatoid arthritis (RA) and 30 with Sjögren's syndrome (SS) were examined. Rim-like immunofluorescence staining was observed in 15 of 31 (48%) sera from patients with PBC, in 1 of 30 with RA and in 1 of 30 with SS. Upon immunoblotting using preparations of whole rat liver nuclear envelopes and their Triton X 100-KCl extract as antigen souces, a 200 kDa protein band was observed in 9 of sera with PBC. Furthermore, upon immunoblotting using the wheat germ aggulutinin-bound fraction of rat liver envelope as antigen, 62, 60 and 54 kDa protein bands corresponding to components of the p62 complex in the nuclear pore complex (Kita et al. Biochem. 113, 377–382) were observed in 7, 5 and 6 samples respectively, of the 31 PBC sera. Our data suggest that PBC sera recognize not only the 210 kDa protein but also the p62 complex proteins.

Key words

anti-gp 210anti-nuclear envelopeanti-nuclear pore complexprimary biliary cirrhosis

Abbreviations

ANA

antinuclear antibody

AMA

anti-mitochondrial antibodies

IF

immunofluorescence

LAP2

lamina-associated polypeptide 2

LBR

lamin B receptor

anti-NBP 60

anti-nuclear localization signal binding protein 60

NE

nuclear envelope

NPC

nuclear pore complex

PBC

primary biliary cirrhosis

RA

rheumatoid arthritis

SLE

systemic lupus erythematosus

SS

Sjögren's syndrome

WGA

wheat germ agglutinin

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • K. Miyachi
    • 1
    • 2
  • M. Shibata
    • 3
  • Y. Onozuka
    • 4
  • F. Kikuchi
  • N. Imai
  • T. Horigome
    • 5
    • 6
  1. 1.Keigu Medical ClinicYokohama, Kanagawa-kenJapan
  2. 2.Health Sciences Research InstituteYokohama, Kanagawa-kenJapan
  3. 3.Tokyo Metropolitan Ebara Hospital, TokyoTokyoJapan
  4. 4.Kawasaki Chuou Hospital, KawasakiKawasakiJapan
  5. 5.Faculty of ScienceNiigata UniversityNiigataJapan
  6. 6.Department of Biochemistry, Faculty of ScienceNiigata UniversityNiigataJapan