Marine Biology

, Volume 1, Issue 3, pp 244–249

Isolation and characterization of a marine bacteriophage

  • W. J. Wiebe
  • J. Liston

DOI: 10.1007/BF00347117

Cite this article as:
Wiebe, W.J. & Liston, J. Marine Biol. (1968) 1: 244. doi:10.1007/BF00347117


A bacteriophage active against a marine Aeromonas sp. was isolated from surface sediments of the North Pacific Ocean at 825 m depth. The sensitive Aeromonas sp. grew between 0° and 33°C but plaques were formed only between 0° and 23°C with a maximum zone of lysis at 5° to 12°C. The phage was rapidly inactivated at temperatures above 45°C. The characteristic plaque formation occurred only on media made with sea-water but some lysis was obtained in distilled water media supplemented with 0.085 M NaCl and 0.05 M MgCl2. Phage replication occurred in cultures of Aeromonas sp. growing under applied hydrostatic pressures of up to 200 atmospheres. Electron microscopy revealed that the phage has an hexagonal head 530 Å in diameter, a thin tail 1200 Å in length and a terminal base plate 400 Å in diameter.

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • W. J. Wiebe
    • 1
  • J. Liston
    • 1
  1. 1.College of FisheriesUniversity of WashingtonSeattleUSA
  2. 2.Department of MicrobiologyUniversity of GeorgiaAthensUSA