Isolation and characterization of a marine bacteriophage
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- Wiebe, W.J. & Liston, J. Marine Biol. (1968) 1: 244. doi:10.1007/BF00347117
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A bacteriophage active against a marine Aeromonas sp. was isolated from surface sediments of the North Pacific Ocean at 825 m depth. The sensitive Aeromonas sp. grew between 0° and 33°C but plaques were formed only between 0° and 23°C with a maximum zone of lysis at 5° to 12°C. The phage was rapidly inactivated at temperatures above 45°C. The characteristic plaque formation occurred only on media made with sea-water but some lysis was obtained in distilled water media supplemented with 0.085 M NaCl and 0.05 M MgCl2. Phage replication occurred in cultures of Aeromonas sp. growing under applied hydrostatic pressures of up to 200 atmospheres. Electron microscopy revealed that the phage has an hexagonal head 530 Å in diameter, a thin tail 1200 Å in length and a terminal base plate 400 Å in diameter.