Skeleton and sclerite formation in the precious red coral Corallium rubrum

Abstract

The carbonate skeleton of the gorgonian coral Corallium rubrum (L.) is composed of both a skeletal axis and numerous sclerites scattered in the mesoglea. Studies carried out on these skeletal elements and their associated tisues using microscopy and X-ray microanalysis, suggest a close relationship between the process of sclerite formation and skeletogenesis. The skeleton is surrounded by an axial epithelium composed of a single cell type. These cells associate intimately with mesogleal sclerites and scleroblasts, incorporating them into a nascent skeleton at the branch tip. Subsequent (sub-apical) growth appears to occur solely through the agency of the axis epithelial cells that serve to physically separate mesogleal sclerites and scleroblasts from contact with the axis. The epithelium is associated with the production of layered calcite crystals and irregular protuberances that constitute the mature, calcareous skeleton. Free sclerites in the mesoglea appear to be the product of multiple cells that are cytologically indistinguishable from those in the axis epithelium. Like the axis, sclerites are produced as layers of calcite crystals with irregular protuberances. The protuberances differ only slightly from those of the axis, and the skeleton is mineralogically indistinguishable from the sclerites. Thus, the skeleton of red coral is not primarily the product of fused sclerites. Instead, we suggest that the axis epithelium treats the incipient skeleton as if it were the core of a single sclerite, and conversely, that the mesogleal scleroblasts of C. rubrum constitute a fragmented axis epithelium.