Molecular and General Genetics MGG

, Volume 215, Issue 2, pp 250–256

Transcriptional organization of the DNA region controlling expression of the K99 gene cluster

  • Bert Roosendaal
  • Jan Damoiseaux
  • Wilco Jordi
  • Frits K. de Graaf
Article

DOI: 10.1007/BF00339725

Cite this article as:
Roosendaal, B., Damoiseaux, J., Jordi, W. et al. Mol Gen Genet (1989) 215: 250. doi:10.1007/BF00339725

Summary

The transcriptional organization of the K99 gene cluster was investigated in two ways. First, the DNA region, containing the transcriptional signals was analyzed using a transcription vector system with Escherichia coli galactokinase (GalK) as assayable marker and second, an in vitro transcription system was employed. A detailed analysis of the transcription signals revealed that a strong promoter PA and a moderate promoter PB are located upstream of fanA and fanB, respectively. No promoter activity was detected in the intercistronic region between fanB and fanC. Factor-dependent terminators of transcription were detected and are probably located in the intercistronic region between fanA and fanB (T1), and between fanB and fanC (T2). A third terminator (T3) was observed between fanC and fanD and has an efficiency of 90%. Analysis of the regulatory region in an in vitro transcription system confirmed the location of the respective transcription signals. A model for the transcriptional organization of the K99 cluster is presented. Indications were obtained that the trans-acting regulatory polypeptides FanA and FanB both function as anti-terminators. A model for the regulation of expression of the K99 gene cluster is postulated.

Key words

ETEC K99 fimbriae fan operon Transcription regulation Anti-terminator 

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Bert Roosendaal
    • 1
  • Jan Damoiseaux
    • 1
  • Wilco Jordi
    • 1
  • Frits K. de Graaf
    • 1
  1. 1.Department of Molecular Microbiology, Biological LaboratoryVrije UniversiteitAmsterdamThe Netherlands

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