Molecular and General Genetics MGG

, Volume 215, Issue 2, pp 231–238

Molecular cloning of resistance genes and architecture of a linked gene cluster involved in biosynthesis of oxytetracycline by Streptomyces rimosus

  • Michael J. Butler
  • Eric J. Friend
  • Iain S. Hunter
  • Frank S. Kaczmarek
  • David A. Sugden
  • Melvyn Warren
Article

DOI: 10.1007/BF00339722

Cite this article as:
Butler, M.J., Friend, E.J., Hunter, I.S. et al. Mol Gen Genet (1989) 215: 231. doi:10.1007/BF00339722

Summary

The isolation of mutants of Streptomyces rimosus which were blocked in oxytetracycline (OTC) production was described previously. The genes for the early steps of antibiotic biosynthesis mapped together. Genomic DNA fragments of S. rimosus which conferred resistance to OTC and complemented all of these non-producing mutants have been cloned. The cloned DNA is physically linked within approximately 30 kb of the genome of S. rimosus. The gene cluster is flanked at each end by a resistance gene each of which, independently, can confer resistance to the antibiotic. In OTC-sensitive strains of S. rimosus, the entire gene cluster including both resistance genes has been deleted. Complementation of blocked mutants by cloned DNA fragments in multi-copy vectors was often masked by a secondary effect of switching off antibiotic productions in strains othersise competent to produce OTC. This adverse effect on OTC production was not observed with recombinants using low copy-number vectors.

Key words

Streptomyces rimosus Antibiotic production Oxytetracycline biosynthetic gene cluster Oxytetracycline resistance 

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Michael J. Butler
    • 1
  • Eric J. Friend
    • 1
    • 2
  • Iain S. Hunter
    • 1
    • 2
  • Frank S. Kaczmarek
    • 2
  • David A. Sugden
    • 1
  • Melvyn Warren
    • 1
  1. 1.International Process Development GroupPfizer LimitedSandwichUK
  2. 2.Molecular GeneticsPfizer Central ResearchGrotonUSA
  3. 3.Institute of GeneticsUniversity of GlasgowGlasgowUK

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