Molecular and General Genetics MGG

, Volume 205, Issue 3, pp 550–556

Analysis of Tn7 transposition

  • Mark Rogers
  • Nelly Ekaterinaki
  • Elaine Nimmo
  • David Sherratt
Original Articles

DOI: 10.1007/BF00338097

Cite this article as:
Rogers, M., Ekaterinaki, N., Nimmo, E. et al. Mol Gen Genet (1986) 205: 550. doi:10.1007/BF00338097

Summary

Five gene products required for Tn7 transposition were identified using genetic complementation tests. Four of these (tns A, tnsB, tnsC and tnsD) are essential for insertion into the attachment site, whereas the fifth (tnsE) is required for transposition to plasmids which lack this site. tnsD is not required for transposition to plasmids lacking the attachment site. This analysis used a chloramphenicol-resistant ‘mini’ transposon containing Tn7 termini but no complete Tn7 gene product and several compatible expression vectors containing cloned Tn7 fragments. The use of transcriptional and translational fusions allowed the identification of two promoters (P1 and P2) at the right-hand end of the transposon and indicated that at least tnsA, tnsB and tnsC are translated in the same direction. Expression from P1 appears to be repressed by tnsB.

Key words

TransposonTn7Genetic complementationExpression vector

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Mark Rogers
    • 1
  • Nelly Ekaterinaki
    • 1
  • Elaine Nimmo
    • 1
  • David Sherratt
    • 1
  1. 1.Institute of GeneticsGlasgow UniversityGlasgowGreat Britain