Article

Molecular and General Genetics MGG

, Volume 210, Issue 1, pp 69-76

First online:

A genetic map of Phycomyces blakesleeanus

  • Margarita OrejasAffiliated withDepartamento de Genética, Facultad de Biología, Universidad de Salamanca
  • , Maria Isabel PeláezAffiliated withDepartamento de Genética, Facultad de Biología, Universidad de León
  • , Maria Isabel AlvarezAffiliated withDepartamento de Genética, Facultad de Biología, Universidad de Salamanca
  • , Arturo P. EslavaAffiliated withDepartamento de Genética, Facultad de Biología, Universidad de Salamanca

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Summary

Complementation tests among Phycomyces auxotrophic strains revealed the existence of four genes with mutants requiring riboflavin, three genes with purine auxotrophs, two with nicotinic acid auxotrophs, and two with lysine auxotrophs. A total of 134 sexual crosses between strains carrying mutations affecting phototropism (madA-madE), carotenoid biosynthesis (carA), auxotrophy (ribA-ribD, purA-purC, lysA and lysB, nicA and nicB, and leuA) and resistance to 5-fluorouracil (furA and furB) were studied; mating type (sex) was also included as a marker. The results from random spore analysis, tetrad analysis, and gene-centromere distances shows that these markers are distributed into 11 linkage groups.

Key words

Phycomyces Genetic map Tetrad analysis