Molecular and General Genetics MGG

, Volume 214, Issue 2, pp 263–270

Yeast nuclear gene CBS2, required for translational activation of cytochrome b, encodes a basic protein of 45 kDa

  • Uwe Michaelis
  • Tobias Schlapp
  • Gerhard Rödel
Article

DOI: 10.1007/BF00337720

Cite this article as:
Michaelis, U., Schlapp, T. & Rödel, G. Mol Gen Genet (1988) 214: 263. doi:10.1007/BF00337720

Summary

In yeast, synthesis of apocytochrome b from mitochondrial COB mRNA depends on at least three nuclear gene products. The translation stimulatory effect by two of these nuclear genes, CBS1 and CBS2, is mediated by the 5′-untranslated leader of COB mRNA. In this report, we show that CBS2 is located on chromosome IV and provide genetic evidence that the CBS2 gene encodes a polypeptide. Determination of the DNA sequence reveals a contiguous open reading frame of 1167 bp. The deduced polypeptide has a calculated molecular weight of 44.5 kDa and is characterized by a high content of positively charged amino acids. It has no significant homology to any known protein. The CBS2 gene is transcribed into low abundance mRNA species with a major transcription initiation site located 97 bp upstream from the ATG start codon next to a poly(dA-dT) stretch.

Key words

DNA sequencePET geneSaccharomyces cerevisiaeTranscription initiationTranslation activator

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Uwe Michaelis
    • 1
  • Tobias Schlapp
    • 1
  • Gerhard Rödel
    • 1
  1. 1.Institut für Genetik und Mikrobiologie der Universität MünchenMünchen 19Federal Republic of Germany
  2. 2.Labor für Molekulare Biologie und Allgemeine PathologieInstitut für Pathologie und Rechtsmedizin der Universität UlmMünchen 45Federal Republic of Germany