Chromosoma

, Volume 100, Issue 1, pp 32–36

The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei

  • Douglas K. Palmer
  • Kathleen O'Day
  • Robert L. Margolis
Chromosoma Focus

DOI: 10.1007/BF00337600

Cite this article as:
Palmer, D.K., O'Day, K. & Margolis, R.L. Chromosoma (1990) 100: 32. doi:10.1007/BF00337600

Abstract

The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic and testes specific histones with protamines. Indirect immunofluorescence studies further show that CENP-A is retained in sperm nuclei in discrete foci, rather than being dispersed throughout the sperm head. These observations suggest that CENP-A is a functionally important component of centromeres, and that pre-existing CENP-A: DNA interactions are likely to be important in organizing the centromeres of the paternal genome during early embryogenesis.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Douglas K. Palmer
    • 1
  • Kathleen O'Day
    • 1
  • Robert L. Margolis
    • 1
  1. 1.Fred Hutchinson Cancer Research CenterSeattleUSA