Biology and Fertility of Soils

, Volume 9, Issue 3, pp 268-272

First online:

Chitinolytic and proteolytic activity of streptomycetes isolated from root-free soil, rhizosphere and mycorrhizosphere of pine (Pinus sylvestris L.)

  • E. StrzelczykAffiliated withLaboratory of Microbiology, Institute of Biology, Nicolaus Copernicus University
  • , K. GorlachAffiliated withLaboratory of Agricultural Microbiology, Faculty of Agriculture, Agricultural and Technical University
  • , H. RózyckiAffiliated withLaboratory of Microbiology, Institute of Biology, Nicolaus Copernicus University

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We tested 75 strains of Streptomyces spp. (25 taken from each environment of soil, rhizosphere, and mycorrhizosphere of pine, Pinus sylvestris L.) and all exhibited chitinolytic activity and hydrolysed gelatine and sodium caseinate in agar media. Enrichment of these media with glucose and NH4NO3 caused induction or stimulation of proteolytic Streptomyces spp. strains (80%) derived from root-free soil; inhibition of this activity was observed in most strains (92%) isolated from the root zone. The post-culture liquids of the rhizosphere strains cultured in the absence of glucose revealed a significantly higher proteolytic activity than those obtained from the root-free soil. The addition of glucose to the medium stimulated proteolytic activity in the post-culture broth of Streptomyces strains derived from soil and the mycorrhizosphere.

Key words

Streptomycetes Chitinolytic Proteolytic activity Soil Pine roots Rhizosphere Pinus sylvestris