Current Genetics

, Volume 20, Issue 6, pp 449–452

Isolation and genetic study of p-fluoro-dl-phenylalanine-resistant mutants overproducing β-phenethyl-alcohol in Saccharomyces cerevisiae

  • Kazuro Fukuda
  • Makoto Watanabe
  • Kozo Asano
  • Kozo Ouchi
  • Seigo Takasawa
Original Articles

DOI: 10.1007/BF00334770

Cite this article as:
Fukuda, K., Watanabe, M., Asano, K. et al. Curr Genet (1991) 20: 449. doi:10.1007/BF00334770

Summary

p-Fluoro-dl-phenylalanine (PFP)-resistant mutants which produce a large amount of β-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, tyr1-pfp, caused both PFP resistance and β-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-pfp DNA.

Key words

YeastMutantp-Fluoro-dl-phenylalanineβ-Phenethyl-alcohol

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Kazuro Fukuda
    • 1
  • Makoto Watanabe
    • 1
  • Kozo Asano
    • 1
  • Kozo Ouchi
    • 2
  • Seigo Takasawa
    • 1
  1. 1.Foods and Liquors Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.IbarakiJapan
  2. 2.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.TokyoJapan