Article

Molecular and General Genetics MGG

, Volume 203, Issue 2, pp 237-244

Molecular analysis of the waxy locus of Zea mays

  • Ralf Bernd KlösgenAffiliated withMax-Planck-Institut für Züchtungsforschung
  • , Alfons GierlAffiliated withMax-Planck-Institut für Züchtungsforschung
  • , Zsuzsanna Schwarz-SommerAffiliated withMax-Planck-Institut für Züchtungsforschung
  • , Heinz SaedlerAffiliated withMax-Planck-Institut für Züchtungsforschung

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Summary

The structure of the wild-type waxy (wx +) locus was determined by sequence analysis of both a genomic and an almost full-size cDNA clone. The coding region comprises 3,718 bp and is composed of 14 exons and 13 small introns. The exons and the promoter region are G/C rich (60%–80%). All three waxy transcripts analysed so far reveal different polyadenylation sites and corresponding polyadenylation signals. The smallest of these mRNAs has a size of 2,263 nucleotides. Northern blot analysis suggests that the tissue-specific expression of the locus is due to transcriptional control. The insertion sites of all transposable element induced waxy mutations analysed have been mapped precisely within the locus. N-terminal sequencing of the mature wx + protein leads to the identification of a maize amyloplast-specific transit peptide of 72 aminoacid residues.

Key words

Gene structure DNA sequence 5′ mapping of mRNA Transit peptide Transposable elements