Molecular and General Genetics MGG

, Volume 206, Issue 2, pp 200–206

Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology

  • Mark A. Estelle
  • Chris Somerville

DOI: 10.1007/BF00333575

Cite this article as:
Estelle, M.A. & Somerville, C. Mol Gen Genet (1987) 206: 200. doi:10.1007/BF00333575


Mutant lines of Arabidopsis thaliana resistant to the artificial auxin 2,4-dichloro phenoxyacetic acid (2,4-D) were isolated by screening for growth of seedlings in the presence of toxic levels of 2,4-D. Genetic analysis of these resistant lines indicated that 2,4-D resistance is due to a recessive mutation at a locus we have designated Axr-1. Mutant seedlings were resistant to approximately 50-fold higher concentrations of 2,4-D than wild-type and were also resistant to 8-fold higher concentrations of indole-3-acetic acid (IAA) than wild-type. Labelling studies with (14C)2,4-D suggest that resistance was not due to changes in uptake or metabolism of 2,4-D. In addition to auxin resistance the mutants have a distinct morphological phenotype including alterations of the roots, leaves, and flowers. Genetic evidence indicates that both auxin resistance and the morphological changes are due to the same mutation. Because of the pleiotropic morphological effects of these mutations the Axr-1 gene may code for a function involved in auxin action in all tissues of the plant.

Key words

Auxin resistance 2,4-Dichloro phenoxyacetic acid Phytohormone Plant development Herbicide-resistance 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Mark A. Estelle
    • 1
  • Chris Somerville
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  2. 2.Department of BiologyIndiana UniversityBloomingtonUSA

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