Molecular and General Genetics MGG

, Volume 126, Issue 1, pp 53–69

Isolation and characterization of a ribonuclease III deficient mutant of Escherichia coli

  • Peter Kindler
  • Till Uwe Keil
  • Peter Hans Hofschneider
Article

DOI: 10.1007/BF00333481

Cite this article as:
Kindler, P., Keil, T.U. & Hofschneider, P.H. Molec. gen. Genet. (1973) 126: 53. doi:10.1007/BF00333481

Summary

A mutant of E. coli K 12 AB301 RNAase19-, selected for its inability to degrade double-stranded RNA, has been isolated and shown to have less than 1% of RNAase III-activity related to the parental strain.

Abbreviations

TCA

trichloracetic acid

RF

replicative form of phage-RNA

Enzymes

Lysozyme

(E.C. 3.2.1.17)

RNAase

(E.C. 2.7.7.16)

DNAase

(E.C. 3.1.4.5)

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Peter Kindler
    • 1
  • Till Uwe Keil
    • 1
  • Peter Hans Hofschneider
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried bei MünchenGermany