Molecular and General Genetics MGG

, Volume 202, Issue 3, pp 394–402

Mitotic recombination between dispersed but related rRNA genes of Schizosaccharomyces pombe generates a reciprocal translocation

Authors

  • Philippe Szankasi
    • Institute of General MicrobiologyUniversity of Bern
  • Christof Gysler
    • Institute of General MicrobiologyUniversity of Bern
  • Ulrich Zehntner
    • Institute of General MicrobiologyUniversity of Bern
  • Urs Leupold
    • Institute of General MicrobiologyUniversity of Bern
  • Jürg Kohli
    • Institute of General MicrobiologyUniversity of Bern
  • Peter Munz
    • Institute of General MicrobiologyUniversity of Bern
Article

DOI: 10.1007/BF00333268

Cite this article as:
Szankasi, P., Gysler, C., Zehntner, U. et al. Molec Gen Genet (1986) 202: 394. doi:10.1007/BF00333268

Summary

Recombination between dispersed yet related serine tRNA genes of Schizosaccharomyces pombe does occur during mitosis but it is approximately three orders of magnitude less frequent than in meiosis. Two mitotic events have been studied in detail. In the first, a sequence of at least 18 nucleotides has been transferred from the donor sup3 gene on the right arm of chromosome I to the related acceptor gene sup12 on the left arm of the same chromosome, thereby leading to the simultaneous change of 8 bp in the acceptor gene. This event must be explained in terms of recombination rather than mutation. It is assumed that it represents mitotic gene conversion, although it was not possible to demonstrate that the donor gene had emerged unchanged from the event. The second case reflects an interaction between sup9 on chromosome III and sup3 on chromosome I. Genetic and physical analysis allows this event to be described as mitotic gene conversion associated with crossingover. The result of this event is a reciprocal translocation. No further chromosomal aberrations were found among an additional 700 potential intergenic convertants tested. Thus intergenic conversion is much less frequently associated with crossingover than allelic conversion. However, the rare intergenic conversion events associated with crossingover provide a molecular mechanism for chromosomal rearrangements.

Key words

Mitotic recombinationSchizosaccharomyces pombe

Copyright information

© Springer-Verlag 1986