Molecular and General Genetics MGG

, Volume 188, Issue 1, pp 135–138

Repair of psoralen-induced crosslinks in cells multiply infected with SV40

  • Jennifer Dean Hall
Article

DOI: 10.1007/BF00333007

Cite this article as:
Hall, J.D. Molec Gen Genet (1982) 188: 135. doi:10.1007/BF00333007

Summary

Experiments were conducted to study the relationship between the production of interstrand crosslinks by 4,5′,8-trimethylpsoralen (psoralen) in simian virus 40 DNA and the ability of psoralen to inactivate the virus. Under conditions where only single viral particles enter a given host cell, approximately one crosslink was lethal to the virus and could not be repaired. In contrast, when multiple viral genomes infected a host cell, psoralen-induced crosslinks were repaired (multiplicity reactivation). A model is proposed for multiplicity reactivation which involves genetic recombination between damaged viral genomes.

Abbreviations used

DNA

deoxyribonucleic acid

SV40

simian virus 40

psoralen

4,5′,8-trimethylpsoralen

EDTA

disodium ethylenediamine-tetraacetate

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Jennifer Dean Hall
    • 1
  1. 1.Department of Cellular and Developmental BiologyUniversity of ArizonaTucsonUSA

Personalised recommendations