Molecular and General Genetics MGG

, Volume 160, Issue 3, pp 325–330

Generalized recombination in tandem duplications of bacteriophage lambda

  • John B. Hays
  • Robert J. Zagursky
Article

DOI: 10.1007/BF00332976

Cite this article as:
Hays, J.B. & Zagursky, R.J. Molec. Gen. Genet. (1978) 160: 325. doi:10.1007/BF00332976

Summary

Recombination between the tandem duplicated segments of λb221a106-15 yields unduplicated (“single-copy”) λb221 phage. The apparent frequency of intramolecular events among these recombinations was determined for both cellular (“Rec”) and bacteriophage (“Red”) generalized recombination systems. The progeny from single-cycle growth experiments with genetically marked duplication phages were treated with EDTA to inactivate all but the singlecopy phages produced by recombination. Analysis of the genotypes of the EDTA-resistant phages suggested that intramolecular events were about 1 to 5 times as frequent as intermolecular ones. While the results suggest that intramolecular events are not intrinsically forbidden, the quantitative values for the ratio depend on the assumption that intracellular phage chromosomes are completely mixed.

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • John B. Hays
    • 1
  • Robert J. Zagursky
    • 1
  1. 1.Department of ChemistryUniversity of Maryland, Baltimore CountyCatonsvilleUSA