Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation
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- Wirth, R., Friesenegger, A. & Fiedler, S. Mol Gen Genet (1989) 216: 175. doi:10.1007/BF00332248
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We have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria — 15 different species belonging to 11 different genera — including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, photosynthetic bacteria and strains for which transformation could not be achieved, up to now, by other methods.