Short Communications

Molecular and General Genetics MGG

, Volume 216, Issue 1, pp 175-177

Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation

  • Reinhard WirthAffiliated withLehrstuhl für Mikrobiologic der Universität München
  • , Anita FrieseneggerAffiliated withLehrstuhl für Mikrobiologic der Universität München
  • , Stefan FiedlerAffiliated withLehrstuhl für Mikrobiologic der Universität München

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Summary

We have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria — 15 different species belonging to 11 different genera — including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, photosynthetic bacteria and strains for which transformation could not be achieved, up to now, by other methods.

Key words

Transformation Electroporation Gram-negative bacteria pKT231