Article

Molecular and General Genetics MGG

, Volume 216, Issue 1, pp 37-43

First online:

Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene

  • Marion SchulzeAffiliated withInstitut für Genetik und Mikrobiologie der Universität München
  • , Gerhard RödelAffiliated withInstitut für Genetik und Mikrobiologie der Universität München

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Summary

The yeast nuclear SCO1 gene is required for accumulation of the mitochondrially synthesized cytochrome c oxidase subunits I and II (COXI and COXII). We cloned and characterized the SCO1 gene. It codes for a 0.9 kb transcript. DNA sequence analysis predicts a 33 kDa protein. As shown by in vitro transcription and translation experiments in combination with import studies on isolated mitochodria, this protein is matured into a 30 kDa polypeptide which is tightly associated with a mitochondrial membrane. The possible function of the SCO1 gene product in the assembly of cytochrome c oxidase is discussed.

Key words

DNA sequence PET gene Saccharomyces cerevisiae Mitochondrial import cytochrome c oxidase