Molecular and General Genetics MGG

, Volume 216, Issue 1, pp 37–43

Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene

  • Marion Schulze
  • Gerhard Rödel

DOI: 10.1007/BF00332228

Cite this article as:
Schulze, M. & Rödel, G. Mol Gen Genet (1989) 216: 37. doi:10.1007/BF00332228


The yeast nuclear SCO1 gene is required for accumulation of the mitochondrially synthesized cytochrome c oxidase subunits I and II (COXI and COXII). We cloned and characterized the SCO1 gene. It codes for a 0.9 kb transcript. DNA sequence analysis predicts a 33 kDa protein. As shown by in vitro transcription and translation experiments in combination with import studies on isolated mitochodria, this protein is matured into a 30 kDa polypeptide which is tightly associated with a mitochondrial membrane. The possible function of the SCO1 gene product in the assembly of cytochrome c oxidase is discussed.

Key words

DNA sequencePET geneSaccharomyces cerevisiaeMitochondrial importcytochrome c oxidase

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Marion Schulze
    • 1
  • Gerhard Rödel
    • 1
  1. 1.Institut für Genetik und Mikrobiologie der Universität MünchenMünchen 19Germany
  2. 2.Labor für Molekulare Biologie und Allgemeine PathologieInstitut für Pathologie und Rechtsmedizin der Universität UlmMünchen 45Germany