, Volume 186, Issue 2, pp 298-300

Plasmid pKM101-mediated mutagenesis in Escherichia coli is inducible

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

A tif-1 umuC36 double mutant of Escherichia coli was constructed. It has been found that the umuC36 mutation prevents both increased spontaneous mutagenesis and enhanced reactivation of UV-irradiated λ, phenomena normally observed in the tif-1 strain grown at 42°C. When the plasmid pKM101 was introduced into tif-1 umuC36, an elevated spontaneous reversion rate of the his-4 mutation observed at 30°C was further increased 6-fold at 42°C. This was accompanied by a 10-fold increase in the ability of tif-1umuC36 containing pKM101 and grown before infection at 42°C to reactivate UV-irradiated λ.

Communicated by H. Böhme