Skip to main content
SpringerLink
Log in

L'ultrastructure de l'adénohypophyse du crapaud Bufo bufo L.

II. Etude de l'évolution des cellules glycoprotidiques de type II après thyroïdectomie chirurgicale

The ultrastructure of the adenohypophysis in the toad, Bufo bufo L.

II. On the formation of glycoprotein containing cells of type II (perivascular basophils) after thyroidectomy

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

In the toad's adenohypophysis, the glycoprotein containing cells of type II (perivascular basophils) undergo important modifications after surgical thyroidectomy. They seem also to be responsible for the production of the thyroid-stimulating-hormone (TSH).

These cells, which become chromophobic and hypertrophie (thyroidectomy cells) after an intense release of the secretory granules, show a great development of the ergastoplasm and a notable extension of the Golgi complex.

The heavy release of the secretory products from the glycoprotein containing cells of type II may take place by intracytoplasmic lysis without destruction of the granule's limiting membrane.

Résumé

Les cellules glycoprotidiques de type II (cyanophiles, palissadiques, périvasculaires) de l'adénohypophyse du Crapaud subissent d'importantes modifications après thyroïdectomie chirurgicale. Elles paraissent donc responsables de la fonction thyréotrope (TSH).

Ces cellules se transforment en cellules chromophobes hypertrophiées (cellules de thyroïdectomie) à la suite d'une dégranulation massive, une hypertrophie importante de l'ergastoplasme qui envahit tout le cytoplasme et une extension appréciable de l'appareil de Golgi.

La libération massive des produits de sécrétion des cellules glycoprotidiques de type II se ferait principalement par lyse intracytoplasmique des granules sans destruction de leur membrane limitante.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Bibliographie

  • Aplington, H. W., Jr.: Cellular changes in the pituitary of Necturus following thyroidectomy. Anat. Rec. 143, 133–146 (1962).

    Google Scholar 

  • Barbieri, F. D.: El ciclo histofisiologico annual en la hipofisis, tiroides y gonadas de la Rana criolla, Leptodactylus chaquensis. Cei. Arch. Farm. Bioquim. Tucumán. 7, 267–324 (1956).

    Google Scholar 

  • Bargmann, W., Hehn, G. v., Lindner, E.: Über die Zellen des braunen Fettgewebes und ihre Innervation. Z. Zellforsch. 85, 601–613 (1968).

    Google Scholar 

  • Barnes, B. G.: The fine structure of the mouse adenohypophysis in various physiological states. In: Cytologie de l'adénohypophyse, ed. J. Benoît, et C. Da Lage, p. 91–110. Paris: Editions du CNRS 1963.

    Google Scholar 

  • Bunt, A. H.: Fine structure of the pars distalis and interrenals of Taricha torosa after administration of metopirone (SU 4885). Gen. comp. Endocr. 12, 134–147 (1969).

    Google Scholar 

  • Cardell, R. R., Jr.: The origin of the thyroidectomy cell in the Salamander. In: Fifth internat. congr. for electron microscopy, Philadelphia, vol. 2, WW3. New York and London: Academic Press 1962.

    Google Scholar 

  • —: Ultrastructure of the salamander thyroidectomy cell. J. Ultrastruct. Res. 10, 515–527 (1964).

    Google Scholar 

  • Cordier, R.: Sur l'aspect histologique et cytologique de l'hypophyse pendant la métamorphose chez Xenopus laevis. C. R. Soc. Biol. (Paris) 148, 845–847 (1948).

    Google Scholar 

  • —: L'hypophyse de Xenopus. Interprétation histophysiologique. Ann. Soc. roy. zool. Belg. 84 1–16 (1953a).

    Google Scholar 

  • —: Cytologie hypophysaire et sa signification fonctionnelle chez l'Amphibien Xenopus. C. R. Ass. Anat. 79, 484–490 (1953b).

    Google Scholar 

  • —, Herlant, M.: Etudes histochimiques sur les cellules du lobe antérieur de l'hypophyse de Xenopus laevis. Ann. Histochim. 2, 349–359 (1957).

    Google Scholar 

  • Dent, J. N.: Seasonal and sexual variation in the pituitary gland of Triturus viridescens. Anat. Rec. 141, 85–95 (1961a).

    Google Scholar 

  • —: Cytological response of the newt pituitary gland to thyroidal depression. Gen. comp. Endocr. 1, 218–231 (1961b).

    Google Scholar 

  • Dingemans, K. P.: On the origin of thyroidectomy cells. In: Fourth european regional conference on electron microscopy Rome, vol. 2, p. 357–358. Tipografia polyglotta vatieana. D. S. Bocciarelli 1968.

  • —: On the origin of thyroidectomy cells. J. Ultrastruct. Res. 26, 480–500 (1969).

    Google Scholar 

  • Doerr-Schott, J.: Modifications ultrastructurales des cellules thyréotropes de l'hypophyse distale de la Grenouille rousse après thyroïdectomie. C. R. Acad. Sci. (Paris) 262, 1973–1976 (1966a).

    Google Scholar 

  • - Cytologie et cytophysiologie de l'adénohypophyse des Amphibiens. Thèse, Strasbourg, enregistrée au centre de documentation du CNRS sous le n∘ A.O. 2049 (1966b).

  • —: Cytologie et cytophysiologie de l'adénohypophyse des Amphibiens. Ann. Biol. 7, 189–225 (1968a).

    Google Scholar 

  • —: Développement de l'hypophyse de Rana temporaria L. Etude au microscope électronique. Z. Zellforsch. 90, 616–645 (1968b).

    Google Scholar 

  • Farquhar, M. G.: Origin and fate of secretory granules in cells of the anterior pituitary gland. Trans. N. Y. Acad. Sci. 23, 346–351 (1961).

    Google Scholar 

  • —: Lysosome function in regulating secretion: disposal of secretory granules in cells of the anterior pituitary gland. In: Lysosomes in biology and pathology (J. T. Dingle and H. B. Fell, ed.), vol. 2, p. 462–482. Amsterdam-London: North-Holland Publishing company 1969.

    Google Scholar 

  • —, Rinehart, J. F.: Cytologic alterations in the anterior pituitary gland following thyroidectomy: an electron microscopic study. Endocrinology 55, 857–876 (1954).

    Google Scholar 

  • Forssmann, W. G., Orci, L.: Zur Endokrinologie der Pylorusschleimhaut. I. Experimentelle Beeinflußung der Gastrin-Zelle. In: 15. Symposium Deutsch. Ges. Endokrinologie (J. Kracht, ed.), vol. 15, p. 408–411. Berlin-Heidelberg-New York: Springer 1969a.

    Google Scholar 

  • —: Ultrastructure and secretory cycle of the gastrin-producing cell. Z. Zellforsch. 101, 419–432 (1969b).

    Google Scholar 

  • Greenawalt, J. W., Foster, G. V., Lehninger, A. L.: The observation of unusual membranous structures associated with liver mitochondria in thyrotoxic rats. In: Fifth internat. congr. for electron microscopy, Philadelphia, vol. 2, p. 00–5. New York and London: Academic Press 1962.

    Google Scholar 

  • Grobstein, C.: Appearance of vacuolated cells in the hypophysis of Triturus torosus following bilateral thyroidectomy. Proc. Soc. exp. Biol. (N.Y.) 38, 801–803 (1938).

    Google Scholar 

  • Guardabassi, A., Grosso, L.: Il quadro citologico dell'ipofisi di Bufo bufo in seguito a trattamento con tiourea e con propionato di testosterone. Monit. zool. ital. 67, 202–221 (1959).

    Google Scholar 

  • Herlant, M.: Apport de la microscopie électronique à l'étude du lobe antérieur de l'hypophyse. In: Cytologie de l'adénohypophyse (J. Benoît et C. Da Lage, ed.), p. 73–90. Paris: Editions du CNRS 1963.

    Google Scholar 

  • —: The cells of the adenohypophysis and their functional significance. In: International review of cytology (G. H. Bourne and J. F. Danielli, eds.), vol. 17, p. 299–398. New York and London: Academic Press 1964.

    Google Scholar 

  • Iturrizza, F. C.: An electron microscopic study of the Toad pars distalis. Gen. comp. Endocr. 4, 225–232 (1964).

    Google Scholar 

  • Jézéquel, A. M.: Dégénérescence myélinique des mitochondries de foie humain dans un épithélioma du cholédoque et un ictère viral. J. Ultrastruct. Res. 3, 210–215 (1959).

    Google Scholar 

  • Karnovsky, M. J.: Simple method for “staining with lead” at high pH in electron microscopy. J. biophys. biochem. Cytol. 11, 729–732 (1961).

    Google Scholar 

  • Kerr, T.: Histology of the distal lobe of the pituitary of Xenopus laevis Daudin. Gen. comp. Endocr. 5, 232–240 (1965).

    Google Scholar 

  • —: The development of the pituitary in Xenopus laevis Daudin. Gen. comp. Endocr. 6, 303–311 (1966).

    Google Scholar 

  • Kurosumi, K.: Functional classification of cell types of the anterior pituitary gland accomplished by electron microscopy. Arch. histol. jap. 29, 329–362 (1968).

    Google Scholar 

  • —, Matsuzawa, T., Watari, N.: Mitochondrial inclusions in the snake renal tubules. J. Ultrastruct. Res. 16, 269–277 (1966).

    Google Scholar 

  • —, Oota, Y.: Corticotrophs in the anterior pituitary gland of gonadectomized and thyroidectomized rats as revealed by electron microscopy. Endocrinology 79, 808–814 (1966).

    Google Scholar 

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).

    Google Scholar 

  • Lundin, M., Schelin, U.: Light and electron microscopic studies on thyrotrophic pituitary adenomas in the mouse. Lab. Invest. 13, 62–68 (1964).

    Google Scholar 

  • Masur, S. K.: Fine structure of the autotransplanted pituitary in the red Eft, Notophthalmus viridescens. Gen. comp. Endocr. 12, 12–32 (1969).

    Google Scholar 

  • Mazzi, V., Peyrot, A., Anzalone, M. R., Toscano, C.: L'histophysiologie de l'adénohypophyse des Tritons crêtés (Triturus cristatus carnifex Laur.). Z. Zellforsch. 72, 597–617 (1966).

    Google Scholar 

  • Meisenheimer, M.: Die Jahreszyclischen Veränderungen der Schilddrüse von Rana temporaria L., und ihre Beziehungen zur Häutung. Z. wiss. Zool. 148, 261–297 (1936).

    Google Scholar 

  • Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physics. 32, 1637 (1961).

    Google Scholar 

  • Mira-Moser, F.: Histophysiologie de la fonction thyréotrope chez le Crapaud Bufo bufo L. Arch. Anat. (Strasbourg) 52, 87–182 (1969a).

    Google Scholar 

  • —: Action de goîtrigènes sur le tétard de Crapaud Bufo bufo L. Arch. Anat. (Strasbourg) 52, 314–332 (1969b).

    Google Scholar 

  • —: L'ultrastructure de l'adénohypophyse du Crapaud Bufo bufo L. I. Identification des types cellulaires et comparaisons des résultats obtenus avec deux fixateurs différents. Z. Zellforsch. 105, 65–90 (1970).

    Google Scholar 

  • Napolitano, L., Fawcett, D.: The fine structure of brown adipose tissue in the newborn mouse and rat. J. biophys. biochem. Cytol. 4, 685–691 (1958).

    Google Scholar 

  • Oordt, P. G. W. J. van: Cell types in the pars distalis of the Amphibia pituitary. In: Cytologie de l'adénohypophyse (J. Benoît et C. Da Lage, ed.) p. 301–313. Paris: Editions du CNRS 1963.

    Google Scholar 

  • —: Changes in the pituitary of the common Toad Bufo bufo, during metamorphosis and the identification of the thyrotropic cells. Z. Zellforsch. 75, 47–56 (1966).

    Google Scholar 

  • Orci, L., Lambert, A. E., Kanazawa, Y., Renold, A. E., Rouiller, Ch.: Synthèse, stockage et libération de l'insuline. Aspects morphologique et biologique. J. Microscopie 8, 73a (1969a).

  • —: Organ culture of fetal rat pancreas. III. Ultrastructural changes occuring in B cells during stimulation of insulin release. Chemico-Biol. Interactions. 1, 341–359 (1969b).

    Google Scholar 

  • - - Stauffacher, W., Rouiller, Ch., Renold, A. E.: Observations nouvelles sur la séquence ultrastructurelle associée à la synthèse et à la sécrétion d'insuline chez le Rat et la Souris à piquants. 5ème Réunion annuelle de l'Association européenne pour l'étude du Diabète, Montpellier, p. 55 (1969c).

  • - Stauffacher, W., Beaven, D., Lambert, A. E., Renold, A. E., Rouiller, Ch.: Ultrastructural events associated with the action of tolbutamide and glybenclamide on pancreatic B cells in vivo and in vitro. In: Pharmacokinetics and mode of action of oral hypoglycemic agents. Edit. A. Loubatières and A. E. Renold. III rd Capri Conference. Acta diabet. lat. 6, Suppl. 1, 271–374 (1969d).

  • Palade, G. E.: Intracisternal granules in the exocrine cells of the pancreas. J. biophys. biochem. Cytol. 2, 417–422 (1956).

    Google Scholar 

  • Pasteels, J. L., Jr.: Recherches expérimentales sur le rôle de l'hypothalamus dans la différenciation cytologique de l'hypophyse de Pleurodeles waltlii. Arch. Biol. (Liège) 68, 65–114 (1957).

    Google Scholar 

  • Pasteels, J. L., Jr.: Etude expérimentale des différentes catégories d'éléments chromophiles de l'hypophyse adulte de Pleurodeles waltlii, de leur fonction et de leur contrôle par l'hypothalamus. Arch. Biol. (Liège) 71, 409–471 (1960).

    Google Scholar 

  • —: Recherches morphologiques et expérimentales sur la sécrétion de prolactine. Arch. Biol. (Liège) 74, 439–453 (1963).

    Google Scholar 

  • —, Herlant, M.: Les différentes catégories de cellules chromophiles de l'hypophyse d'Amphibiens. Anat. Anz. 109, 764–768 (1960).

    Google Scholar 

  • Pisano, A.: Contributo alla istofisiologia della tiroide di Rana esculenta. Arch. ital. Anat. Embriol. 47, 705–737 (1942).

    Google Scholar 

  • Prieto-Diaz, H. E., Iturriza, F. C., Thea, J. P.: Azocarminophil cells of the toad adenohypophysis after mercaptoimidazol or radiothyroidectomy. Gen. comp. Endocr. 3, 569–578 (1963).

    Google Scholar 

  • Rinehart, J. F., Farquhar, M. G.: Electron microscopic studies of anterior pituitary gland. J. Histochem. Cytochem. 1, 93–113 (1953).

    Google Scholar 

  • Rouiller, Ch., Jézéquel, A. M.: Electron microscopy of the liver. In: The Liver, ed. Ch. Rouiller, vol. 1, p. 195–264. New York and London: Academic Press 1963.

    Google Scholar 

  • Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).

    Google Scholar 

  • Sano, M.: Further studies on the theta cell of the Mouse anterior pituitary as revealed by electron microscopy, with special reference to the mode of secretion. J. Cell Biol. 15, 85–97 (1962).

    Google Scholar 

  • Saxen, L., Saxen, E., Toivonen, S., Salimaki, K.: The anterior pituitary and the thyroid function during normal and abnormal development of the frog. Ann. Zool. Soc. “ Vanamo” 18, 1–44 (1957a).

    Google Scholar 

  • —: Quantitative investigations on the anterior pituitary thyroid mechanism during metamorphosis. Endocrinology 61, 35–44 (1957b).

    Google Scholar 

  • Schellens, J. P. M., Ossentjuk, E.: Mitochondrial ultrastructure with crystalloid inclusions in an unusual type of human myopathy. Virchows Arch. Abt. B Zellpath. 4, 21–29 (1969)

    Google Scholar 

  • Smith, R. E., Farquhar, M. G.: Lysosome function in the regulation of the secretory process in cells of the anterior pituitary. J. Cell Biol. 31, 319–347 (1966).

    Google Scholar 

  • Suzuki, T., Mostofi, F. K.: Intramitochondrial filamentous bodies in the thick limb of Henle of the rat kidney. J. Cell Biol. 33, 605–623 (1967).

    Google Scholar 

  • Themann, H., Bassewitz, D. B., v.: Parakristalline Einschlußkörper der Mitochondrien des menschlichen Leberparenchyms. Elektronenmikroskopische und histochemische Untersuchungen. Cytobiologie 1, 135–151 (1969).

    Google Scholar 

  • Tixier-Vidal, A.: Caractères ultrastructuraux des types cellulaires de l'adénohypophyse du Canard mâle. Arch. Anat. micr. Morph. exp. 54, 791–780 (1965).

    Google Scholar 

  • Toutain, J.: Action de la thyroïdectomie et du froid sur les cellules thyréotropes de l'hypophyse antérieure de la Grenouille verte, Rana esculenta L. C. R. Acad. Sci. (Paris) 254, 3899–3900 (1962).

    Google Scholar 

  • Watrach, A. M.: Degeneration of mitochondria in lead poisoning. J. Ultrastruct. Res. 10, 177–181 (1964).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut d'Histologie et d'Embryologie, Ecole de Médecine de l'Université de Genève, Genève, Suisse

    Fanny Mira-Moser

Authors
  1. Fanny Mira-Moser
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Travail réalisé avec l'aide du Fonds national suisse de la Recherche scientifique (Crédit No 5344,3).

Nous remercions tout particulièrement Mme Sidler-Ansermet, photographe, et Mme Estoppey, secrétaire, de l'aide qu'elles ont apportée à la réalisation de ce mémoire.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mira-Moser, F. L'ultrastructure de l'adénohypophyse du crapaud Bufo bufo L.. Z. Zellforsch. 112, 266–286 (1971). https://doi.org/10.1007/BF00331844

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00331844

Key-Words

Search

Navigation