Molecular and General Genetics MGG

, Volume 215, Issue 1, pp 58–64

Plants regenerated from auxin-auxotrophic variants are inviable

  • Anne D. Blonstein
  • Tiina Vahala
  • Maarten Koornneef
  • Patrick J. King
Article

DOI: 10.1007/BF00331303

Cite this article as:
Blonstein, A.D., Vahala, T., Koornneef, M. et al. Mol Gen Genet (1988) 215: 58. doi:10.1007/BF00331303

Summary

Shoots regenerated from auxin-auxotrophic variants of Nicotiana plumbaginifolia were inviable when cultured in vitro in the absence of auxin. Variant shoots survived longer when grafted to wild-type stocks but eventually died after a characteristic pattern of degeneration. The auxin auxotrophs were isolated after mutagen treatment by a total isolation method as infrequent variants amongst haploid protoplast-derived cell colonies. The variants responded to several active auxins but, unlike the wild type, not to cytokinin. Plant regeneration from variant cultures ceased at early stages of shoot formation after complete withdrawal of auxin from the medium.

Key words

Auxin-auxotrophNicotiana plumbaginifolia-HaploidProtoplastVariant

Abbreviations

BAP

6-benzylaminopurine

BUdR

5-bromo-2′-deoxyuridine

2,4-D

2,4-dichlorophenoxyacetic acid

DMSO

dimethyl sulfoxide

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

MNNG

N-methyl-N′-nitro-N-nitrosoguanidine

NAA

1-naphthaleneacetic acid

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Anne D. Blonstein
    • 1
  • Tiina Vahala
    • 1
  • Maarten Koornneef
    • 1
  • Patrick J. King
    • 1
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland
  2. 2.Institute of Physiological BotanyUniversity of UppsalaUppasalaSweden
  3. 3.Department of GeneticAgricultural UniversityWageningenThe Netherlands