Molecular and General Genetics MGG

, Volume 215, Issue 1, pp 58-64

First online:

Plants regenerated from auxin-auxotrophic variants are inviable

  • Anne D. BlonsteinAffiliated withFriedrich Miescher-Institut
  • , Tiina VahalaAffiliated withFriedrich Miescher-Institut
  • , Maarten KoornneefAffiliated withFriedrich Miescher-Institut
  • , Patrick J. KingAffiliated withFriedrich Miescher-Institut

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Shoots regenerated from auxin-auxotrophic variants of Nicotiana plumbaginifolia were inviable when cultured in vitro in the absence of auxin. Variant shoots survived longer when grafted to wild-type stocks but eventually died after a characteristic pattern of degeneration. The auxin auxotrophs were isolated after mutagen treatment by a total isolation method as infrequent variants amongst haploid protoplast-derived cell colonies. The variants responded to several active auxins but, unlike the wild type, not to cytokinin. Plant regeneration from variant cultures ceased at early stages of shoot formation after complete withdrawal of auxin from the medium.

Key words

Auxin-auxotroph Nicotiana plumbaginifolia-Haploid Protoplast Variant