Molecular and General Genetics MGG

, Volume 209, Issue 3, pp 563–569

The molecular organization of the lysostaphin gene and its sequences repeated in tandem

  • Peter Heinrich
  • Ralf Rosenstein
  • Maria Böhmer
  • Peter Sonner
  • Friedrich Götz
Article

DOI: 10.1007/BF00331163

Cite this article as:
Heinrich, P., Rosenstein, R., Böhmer, M. et al. Mol Gen Genet (1987) 209: 563. doi:10.1007/BF00331163

Summary

The gene encoding lysostaphin of Staphylococcus staphylolyticus was cloned in Escherichia coli and its DNA sequence was determined. The complete coding region comprises 1440 base pairs corresponding to a precursor of 480 amino acids (molecular weight 51669). It was shown by NH2-terminal amino acid sequence analysis of the purified extracellular lysostaphin from S. staphylolyticus that the mature lysostaphin consists of 246 amino acid residues (molecular weight 26926). Polyacrylamide gel electrophoresis revealed a similar molecular weight for the most active form. By computer analysis the secondary protein structure was predicted. It revealed three distinct regions in the precursor protein: a typical signal peptide (ca. 38 aa), a hydrophilic and highly ordered protein domain with 14 repetitive sequences (296 aa) and the hydrophobic mature lysostaphin. The lysostaphin precursor protein appears to be organized as a preprolysostaphin.

Key words

Lysostaphin gene Preprolysostaphin Repetitive sequences Secondary structure Staphylococcus staphylolyticus 

Abbreviations

aa

amino acid(s)

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Peter Heinrich
    • 1
  • Ralf Rosenstein
    • 3
  • Maria Böhmer
    • 3
  • Peter Sonner
    • 2
  • Friedrich Götz
    • 3
  1. 1.Institut für Biochemie der Universität MünchenMünchen 2Federal Republic of Germany
  2. 2.Zoologisches Institut der Universität MünchenMünchen 2Federal Republic of Germany
  3. 3.Lehrstuhl für MikrobiologieTechnische Universität MünchenMünchen 2Federal Republic of Germany