Molecular and General Genetics MGG

, Volume 204, Issue 3, pp 383–396

The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector

  • Csaba Koncz
  • Jeff Schell
Article

DOI: 10.1007/BF00331014

Cite this article as:
Koncz, C. & Schell, J. Molec Gen Genet (1986) 204: 383. doi:10.1007/BF00331014

Summary

A “plant gene vector cassette” to be used in combination with various Escherichia coli gene-cloning vectors was constructed. This cassette contains a replication and mobilization unit which allows it to be maintained and to be transferred back and forth between E. coli and Agrobacterium tumefaciens hosts provided these hosts contain plasmid RK2 replication and mobilization helper functions. The cassette also harbors a transferable DNA unit with plant selectable marker genes and cloning sites which can be combined with different bacterial replicons, thus facilitating the reisolation of transferred DNA from transformed plants in E. coli. The vector cassette contains two different promoters derived from the T-DNA-encoded genes 5 and nopaline synthase (NOS). By comparing the levels of expression of the marker enzymes linked to each of these promoter sequences, it was found that the gene 5 promoter is active in a tissue-specific fashion whereas this is not the case for the NOS promoter. This observation provides the first documented instance of a gene derived from a procaryotic host the expression of which is apparently regulated by plant growth factors.

Key words

Agrobacterium binary vector systemPlant transformationTissue specificityT-DNA-encoded genes

Abbreviations

OCS

octopine synthase (gene)

NOS

nopaline synthase (gene)

NPT-II

neomycin phosphotransferase (gene) of transposon Tn5

vir

Ti-plasmid region encoding virulence functions

Cb

carbenicillin

Gm

gentamycin

Km

kanamycin

Cm

chloramphenicol

Sm

streptomycin

Sp

spectinomycin

Rif

rifampicin

Ery

erythromycin

bom

basis of mobilization

orir

origin of conjugational plasmid transfer

Tra, Mob

functions required for conjugational transfer of plasmids

BAP

N6-benzylaminopurine

NAA

α-naphthaleneacetic acid

CTAB

N-cetyl-N,N,N-trimethyl-ammonium bromide

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Csaba Koncz
    • 1
    • 2
  • Jeff Schell
    • 2
  1. 1.Institute of GeneticsBiological Research Center of Hungarian Academy of SciencesSzegedHungary
  2. 2.Max-Planck-Institut für ZüchtungsforschungKöln 30Federal Republic of Germany