Molecular and General Genetics MGG

, Volume 197, Issue 2, pp 345–346

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Authors

  • Jef D. Boeke
    • Massachusetts Institute of Technology and Whitehead Institute for Biomedical Research
  • Francois La Croute
    • Laboratoire de Genetique Physiologique
  • Gerald R. Fink
    • Massachusetts Institute of Technology and Whitehead Institute for Biomedical Research
Short Communication

DOI: 10.1007/BF00330984

Cite this article as:
Boeke, J.D., La Croute, F. & Fink, G.R. Mol Gen Genet (1984) 197: 345. doi:10.1007/BF00330984

Summary

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Copyright information

© Springer-Verlag 1984