Molecular and General Genetics MGG

, Volume 217, Issue 1, pp 162–167

In vitro and in vivo studies on the mitochondrial import of CBS1, a translational activator of cytochrome b in yeast

  • Andreas Körte
  • Vera Forsbach
  • Thomas Gottenöf
  • Gerhard Rödel
Article

DOI: 10.1007/BF00330956

Cite this article as:
Körte, A., Forsbach, V., Gottenöf, T. et al. Mol Gen Genet (1989) 217: 162. doi:10.1007/BF00330956

Summary

Translation of mitochondrial cytochrome b mRNA in yeast is activated by the product of the nuclear gene CBS1. CBS1 encodes a 27 kDa precursor protein, which is cleaved to a 24 kDa mature protein during the import into isolated mitochondria. The sequences required for mitochondrial import reside in the amino-terminal end of the CBS1 precursor. Deletion of the 76 amino-terminal amino acids renders the protein incompetent for mitochondrial import in vitro and non-functional in vivo. When present on a high copy number plasmid and under the control of a strong yeast promoter, biological function can be restored by this truncated derivative. This observation indicates that the CBS1 protein devoid of mitochondrial targeting sequences can enter mitochondria in vivo, possibly due to a bypass of the mitochondrial import system.

Key words

Nucleotide sequence PET gene Mitochondrial import Saccharomyces cerevisiae 

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Andreas Körte
    • 1
  • Vera Forsbach
    • 1
  • Thomas Gottenöf
    • 2
  • Gerhard Rödel
    • 2
  1. 1.Institut für Genetik und Mikrobiologie der Universität MünchenMünchen 19Federal Republic of Germany
  2. 2.Labor für Molekulare Biologie und Allgemeine PathologieInstitut für Pathologie und Rechtsmedizin der Universität UlmMünchen 45Federal Republic of Germany