Molecular and General Genetics MGG

, Volume 217, Issue 1, pp 141–148

Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence

  • Jay C. D. Hinton
  • Julie M. Sidebotham
  • Lizbeth J. Hyman
  • Michel C. M. Pérombelon
  • George P. C. Salmond
Article

DOI: 10.1007/BF00330953

Cite this article as:
Hinton, J.C.D., Sidebotham, J.M., Hyman, L.J. et al. Mol Gen Genet (1989) 217: 141. doi:10.1007/BF00330953

Summary

The blackleg pathogen Erwinia carotovora subsp. atroseptica (Eca) causes an economically important disease of potatoes. We selected a genetically amenable Eca strain for the genetic analysis of virulence. Tn5 mutagenesis was used to generate nine mutants which exhibited reduced virulence (Rvi-) of strain SCRI1043. Following physiological characterisation, mutants were divided into three classes: (1) auxotrophs; (2) extracellular enzyme mutants; and (3) a growth rate mutant. The isolation of these Rvi- mutants has allowed us to consider some factors that affect Eca virulence.

Key words

Erwiniaλ::Tn5 mutagenesisMutants of reduced virulencePectolytic enzyme mutants

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Jay C. D. Hinton
    • 1
  • Julie M. Sidebotham
    • 1
  • Lizbeth J. Hyman
    • 2
  • Michel C. M. Pérombelon
    • 2
  • George P. C. Salmond
    • 1
  1. 1.Department of Biological SciencesUniversity of WarwickCoventryEngland
  2. 2.Scottish Crop Research InstituteDundeeScotland