Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli
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- Froman, B.E., Tait, R.C. & Gottlieb, L.D. Mol Gen Genet (1989) 217: 126. doi:10.1007/BF00330951
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The nucleotide sequence of the gene encoding the glycolytic enzyme phosphoglucose isomerase (PGI) from Escherichia coli is presented. The gene encodes a polypeptide of 549 amino acids. The transcriptional start point of the gene was determined and found to lie within a consensus promoter region. The amino acid sequence derived from the E. coli PGI gene can be aligned without insertions or deletions to the predicted amino acid sequence of a nuclear-encoded chloroplast isozyme of PGI from a higher plant, and the two sequences have a similarity of 87.6%. The amino acid sequence similarity between E. coli and that predicted from cDNA sequences for mouse and pig PGI is approximately 65%.
Key wordsGlycolytic enzymeMolecular evolution
open reading frame