Rec-dependent and Rec-independent recombination of plasmid-borne duplication in Escherichia coli K12
- Cite this article as:
- Matfield, M., Badawi, R. & Brammar, W.J. Mol Gen Genet (1985) 199: 518. doi:10.1007/BF00330768
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Plasmidic recombination in E. coli K12 has been previously demonstrated to be dependent on the host rec genotype. The construction of plasmids that carry a duplication within an antibiotic-resistance gene is described. Recombination between the direct repeats recreates an active antibiotic-resistance gene, allowing quantitative analysis of recombination frequencies in a closely related set of E. coli K12 strains carrying various rec mutations. Using this system, intraplasmidic recombination of a duplication within the pBR322 tetracycline-resistance gene is shown to be rec-dependent while recombination of a similar duplication within the kanamycin-resistance gene of Tn903 is shown to be independent of recA, recB, recC, recE, recF and sbcB.