Article

Molecular and General Genetics MGG

, Volume 199, Issue 3, pp 381-387

First online:

Suppressors of temperature-sensitive mutations in a ribosomal protein gene, rpsL (S12), of Escherichia coli K12

  • Hiroko NashimotoAffiliated withInstitute of Medical Science, University of Tokyo
  • , Akiko MiuraAffiliated withInstitute of Medical Science, University of Tokyo
  • , Haruo SaitoAffiliated withInstitute of Medical Science, University of Tokyo
  • , Hisao UchidaAffiliated withInstitute of Medical Science, University of Tokyo

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon. One of these mutations, ts118, resulted in a mutant S12 protein which behaved differently from the wild-type S12 on CM-cellulose column chromatography. Suppressors of these ts mutations were isolated and characterized; one was found to be a mutation of a nonribosomal protein gene which was closely linked to the RNAase III gene on the E. coli chromosome. This suppressor, which was recessive to its wild-type allele, was cloned into a transducing phage and mapped finely. A series of cold-sensitive mutations, affecting the assembly of ribosomes at 20°C, was isolated within the purL to nadB region of the E. coli chromosome and one group, named rbaA, mapped at the same locus as the suppressor mutation, showing close linkage to the RNAase III gene.