Molecular and General Genetics MGG

, Volume 190, Issue 2, pp 355–357

Replication of pBR322 DNA in stringent and relaxed strains of Escherichia coli

  • Michael Hecker
  • Andreas Schroeter
  • Friedrich Mach
Short Communication

DOI: 10.1007/BF00330665

Cite this article as:
Hecker, M., Schroeter, A. & Mach, F. Mol Gen Genet (1983) 190: 355. doi:10.1007/BF00330665

Summary

Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+: CP78; relA: CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated only in the relaxed strain and was inhibited in its stringent counterpart. The results suggest that replication of plasmid DNA is negatively controlled by the relA+ allele.

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Michael Hecker
    • 1
  • Andreas Schroeter
    • 1
  • Friedrich Mach
    • 1
  1. 1.Section of BiologyErnst-Moritz-Arndt-UniversityGreifswaldGerman Democratic Republic