Molecular and General Genetics MGG

, Volume 211, Issue 2, pp 244–251

Cointegrate formation by IS50 requires multiple donor molecules

  • Ann Lichens-Park
  • Michael Syvanen
Article

DOI: 10.1007/BF00330600

Cite this article as:
Lichens-Park, A. & Syvanen, M. Mol Gen Genet (1988) 211: 244. doi:10.1007/BF00330600

Summary

The insertion sequence, IS50R, promotes cointegrate formation between a lambda::IS50R phage and the chromosome of Escherichia coli strain C. We show that formation of cointegrates mediated by IS50R between the non-replicating phage genome and the bacterial chromosome requires multiple donor molecules and depends on homologous recombination functions. We conclude that the two copies of IS50 present in the cointegrate originate in two different molecules. Thus, the existence of the cointegrate structure cannot be used as evidence for replication of IS50 sequences during IS50 transposition.

Key words

IS50 Cointegrate Dimer donor model Bacteriophage lambda Insertion sequence 

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Ann Lichens-Park
    • 1
  • Michael Syvanen
    • 2
  1. 1.Department of Microbiology and Molecular GeneticsHarvard Medical SchoolBostonUSA
  2. 2.Department of Medical Microbiology and Immunology, School of MedicineUniversity of CaliforniaDavisUSA