Molecular and General Genetics MGG

, Volume 218, Issue 1, pp 118–126

Regulatory elements involved in the tissue-specific expression of the yellow gene of Drosophila

  • Mark Martin
  • Yuan B. Meng
  • William Chia
Article

DOI: 10.1007/BF00330574

Cite this article as:
Martin, M., Meng, Y.B. & Chia, W. Mol Gen Genet (1989) 218: 118. doi:10.1007/BF00330574

Summary

We have assessed the DNA sequence requirements for the correct spatial pattern and phenotypic expression of y in the late embryo/larvae. The wild-type larval phenotype requires both the regions between-294 bp and-92 bp and a portion of the intron; the sequence element(s) located within the intron can act in a position independent manner to effect the wild-type larval phenotype. The larval expression pattern was examined by tissue experiments in situ and by staining germline transformants derived from various y/lacZ fusion constructs. The larval expression of y is restricted to the mouthparts, microsetae and anal plates. While the-495 bp to+194 bp region alone cannot effect a wild-type larval expression pattern, this region in conjunction with the intron appears to be sufficient to drive β-gal expression in an essentially wild-type pattern. Our data further suggest that the-294 bp to-92 bp region contains elements which specify the larval pattern and that the element(s) in the intron normally act to enhance the level of expression necessary for the wild-type larval phenotype. We also present a phenotypic analysis of the adult cuticle structures of germline transformants derived from a variety of deletion and rearrangement constructs of the y gene. This analysis has revealed several new features associated with the regulation of y expression.

Key words

DrosophilaRegulationyellow geneGermline transformation

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Mark Martin
    • 1
  • Yuan B. Meng
    • 1
  • William Chia
    • 1
  1. 1.Department of Biochemistry, School of Medical SciencesUniversity of BristolBristolUK