Molecular and General Genetics MGG

, Volume 218, Issue 1, pp 87–92

Isolation of the facA (acetyl-Coenzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans


  • Ruth A. Sandeman
    • Department of GeneticsUniversity of Melbourne
  • Michae J. Hynes
    • Department of GeneticsUniversity of Melbourne

DOI: 10.1007/BF00330569

Cite this article as:
Sandeman, R.A. & Hynes, M.J. Mol Gen Genet (1989) 218: 87. doi:10.1007/BF00330569


Acetate inducible genes of Aspergillus nidulans were cloned via differential hybridization to cDNA probes. Using transformation of mutant strains the genes were identified as facA (acetyl-Coenzyme A synthetase) and acuE (malate synthase). The levels of RNA encoded by these genes were shown to be acetate inducible and subject to carbon catabolite repression. Induction is abolished in a facB mutant and carbon catabolite repression is relieved in a creA mutant.

Key words

Aspergillus nidulansTransformationAcetateMalate synthaseAcetyl-Coenzyme A synthetaseGene regulation

Copyright information

© Springer-Verlag 1989