Molecular and General Genetics MGG

, Volume 202, Issue 1, pp 58–61

Regulation of exoprotein gene expression in Staphylococcus aureus by agr

Authors

  • P. Recsei
    • Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.
  • B. Kreiswirth
    • Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.
  • M. O'Reilly
    • Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.
  • P. Schlievert
    • Department of MicrobiologyUniversity of Minnesota Medical School
  • A. Gruss
    • Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.
  • R. P. Novick
    • Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.
Article

DOI: 10.1007/BF00330517

Cite this article as:
Recsei, P., Kreiswirth, B., O'Reilly, M. et al. Mol Gen Genet (1986) 202: 58. doi:10.1007/BF00330517

Summary

Insertion of the erythromycin-resistance transposon Tn551 into the Staphylococcus aureus chromosome at a site which maps between the purB and ilv loci has a pleiotrophic effect on the production of a number of extracellular proteins. Production of alpha, beta and delta hemolysin, toxic shock syndrome toxin (TSST-1) and staphylokinase was depressed about fifty-fold while protein A production was elevated twenty-fold. Hybridization analysis showed that the defect in expression of TSST-1 and alpha hemolysin was at the transcriptional level. Inability of the mutant strain to express either a cloned TSST-1 gene or the chromosomal gene indicates that the transposon has inactivated a trans-active positive control element. This element has been designated agr for accessory gene regulator.

Key words

Gene regulationPathogenicityExoproteinsPletotropismS. aureus

Copyright information

© Springer-Verlag 1986