Molecular and General Genetics MGG

, Volume 209, Issue 1, pp 122–128

Genetic analysis of the parB+ locus of plasmid R1

  • Poul Baad Rasmussen
  • Kenn Gerdes
  • Søren Molin
Article

DOI: 10.1007/BF00329846

Cite this article as:
Rasmussen, P.B., Gerdes, K. & Molin, S. Mol Gen Genet (1987) 209: 122. doi:10.1007/BF00329846

Summary

Plasmid R1 in Escherichia coli carries two loci which independently contribute to the stable maintenance of the plasmid. A genetic analysis of one of these, parB+, was carried out, and it was shown that the minimal region exerting stabilizing activity comprises at most 580 bp. The nucleotide sequence of the parB+ locus was determined, and indicated the presence of two genes, of which one probably codes for a 52 amino acid polypeptide, whereas the other gene product may be an untranslated RNA. These suggestions, based on the nucleotide sequence information, were supported by gene expression studies employing lac fusions. An incompatibility phenotype connected to parB+ was localized to that par of the 580 bp parB+ region which seems to encode the untranslated RNA.

Key words

Plasmid stability Nucleotide sequence Gene expression 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Poul Baad Rasmussen
    • 1
  • Kenn Gerdes
    • 2
  • Søren Molin
    • 2
  1. 1.Department of Molecular BiologyOdense UniversityOdenseDenmark
  2. 2.Department of MicrobiologyThe Technical University of DenmarkLyngbyDenmark
  3. 3.Nordisk GentofteGentofteDenmark