Molecular and General Genetics MGG

, Volume 209, Issue 2, pp 403–407

Nitrogen regulation of transport operons: Analysis of promoters argTr and dhuA

  • Gudrun Schmitz
  • Peter Dürre
  • Guy Mullenbach
  • Giovanna Ferro-Luzzi Ames
Short Communications

DOI: 10.1007/BF00329673

Cite this article as:
Schmitz, G., Dürre, P., Mullenbach, G. et al. Molec Gen Genet (1987) 209: 403. doi:10.1007/BF00329673


In Salmonella typhimurium the periplasmic permeases for histidine and for lysine-arginine-ornithine are regulated by nitrogen availability. The nature of the dhuA and argTr promoters of the operons coding for these permeases was analyzed by placing the galactokinase gene under their control (in vector pKO-1). argTr was found to respond to nitrogen regulation. We investigated the involvement of a mirror symmetry in argTr in its regulation by nitrogen. It had been postulated previously (Higgins and Ames 1982) that mirror symmetries might act as protein recognition sites important in regulation of gene expression. Here we demonstrate that the mirror symmetry in argTr is not involved in nitrogen control. Contrary to expectation, the galK gene was not regulated by nitrogen when it was placed under dhuA control. Here we propose a possible explanation for this finding.

Key words

Nitrogen regulation Mirror symmetry Periplasmic permease Promoters 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Gudrun Schmitz
    • 1
  • Peter Dürre
    • 2
  • Guy Mullenbach
    • 3
  • Giovanna Ferro-Luzzi Ames
    • 4
  1. 1.Department of Molecular BiologyBoehringer Manheim GmbH, Biochemical Research CenterPenzbergFederal Republic of Germany
  2. 2.Institut für MikrobiologieUniversität GöttingenGöttingenFederal Republic of Germany
  3. 3.Chiron Research LaboratoriesChiron CorporationEmeryvilleUSA
  4. 4.Department of BiochemistryUniversity of CaliforniaBerkeleyUSA

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