Original Articles

Molecular and General Genetics MGG

, Volume 209, Issue 2, pp 374-381

Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis

  • Valerie M. WilliamsonAffiliated withARCO Plant Cell Research Institute
  • , Charlotte E. PaquinAffiliated withARCO Plant Cell Research Institute

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Summary

Insertion of the transposable element Ty at the ADH4 locus results in increased levels of a new alcohol dehydrogenase (ADH) activity in Saccharomyces cerevisiae. The DNA sequence of this locus has been determined. It contains a long open reading frame which is not homologous to the other ADH isozymes that have been characterized in S. cerevisiae nor does it show obvious homology to Drosophila ADH. The hypothetical ADH does, however, show strong homology to the sequence of an iron-activated ADH from the bacterium Zymomonas mobilis. Thus ADH4 appears to encode an ADH structural gene which, along with the Zymomonas enzyme, may define a new family of alcohol dehydrogenases.

Key words

Alcohol dehydrogenase Saccharomyces cerevisiae Zymomonas mobilis Evolution